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cd49d r1-2 antibody  (Thermo Fisher)


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    Thermo Fisher cd49d r1-2 antibody
    Cd49d R1 2 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd49d+r1-2+antibody/anti+cd49f+antibody/pmc11984669-238-51-73
    Average 90 stars, based on 1 article reviews
    cd49d r1-2 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Staining:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Incubation:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Fluorescence:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Expressing:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Comparison:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Transduction:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Control:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    In Vitro:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).

    Binding Assay:

    Article Title: CD301b/MGL2 + Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis
    Article Snippet: Flow cytometry Antibodies with the following specificities were used (clones listed, purchased from BioLegend unless noted otherwise): CD3ε (145-2C11 and 17A2), CD4 (RM4-5 and GK1.5), B220 (RA36B2), Ly6G (1A8), CD11b (M1/70), F4/80 (BM8), CX3CR1 (SA011F11), Ly6C (AL-21, BD), by guest on February 3, 2018 http://circ.ahajournals.org/ D ow nloaded from 10.1161/CIRCULATIONAHA.117.033144 9 CD301b/MGL2 (URA-1), CD64 (X54-5/7.1), CD16.2 (9E9), CD49d (R1-2), Syk (5F5), CD49d (R1-2, eBioscience), CD16/32 (2.4G2).

    Article Title: Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8 + T cell differentiation
    Article Snippet: The following antibodies were used with the indicated specificity and the appropriate combinations of fluorochromes: Thy1.1 (OX-7 or HIS51) and CD62L (MEL-14, both BD Pharmingen), CD8 (53-6.7), CD25 (PC61.5), CD122 (5H4), CD27 (LG.7F9), CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD103 (2E7), CD29 (eBioHMb1-1), CD49d (R1-2), KLRG-1 (2F1), CXCR3 (Cxcr3-173), IFN-γ (XMG1.2), TNF (MP6-XT22), IL-2 (JES6-5H4, all eBioscience) and appropriate isotype controls.

    Article Title: Age‐Dependent Bi‐Phasic Dynamics of Ly49 + CD8 + Regulatory T Cell Population
    Article Snippet: Freshly isolated splenocytes of young, middle‐aged, and old aged mice were stained for surface antigens and incubated for 30 min at 4°C with the following fluorescence dye‐labeled antibodies specific for CD3 (17A2), B220 (RA3‐6B2), CD19 (1D3), CD4 (GK 1.5), CD44 (IM7), PD‐1 (J43), CXCR5 (SPRCL5), CD8β (H35‐17.2), Ly49C/F/I/H (14B11), CD122 (TM‐β1), CD25 (PC61.5), CD49d (R1‐2), Ly49D (4E5), Ly49I (YLi‐90), Ly49E/F (CM4), Ly49G2 (411), Ly49A (YE1), IL‐10 (JES5‐16E3), and NKG2D (CX5) were purchased from Thermo Fisher, Biolegend, and Cytek (Tonbo).

    Article Title: The Endoplasmic Reticulum Chaperone Protein GRP94 Is Required for Maintaining Hematopoietic Stem Cell Interactions with the Adult Bone Marrow Niche
    Article Snippet: Integrin α4 and α5 were detected using CD49d (R1-2, eBioscience) and CD49e (5H10-27, BD Pharmingen).

    Article Title: Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells
    Article Snippet: Antibodies used in cell phenotyping were CD11a (M17/4), CD11b (M1/70), CD11c (N418), CD16/CD32 (93), CD18 (M18/2), CD49d (R1-2), CD51 (RMV-7), CD62L (MEL-14), CD80 (16-10A1), CD86 (GL1), 33D1, Gr-1 (RB6-8C5), F4/80 (BM8) from eBioscience (San Diego, CA); CD49a (Ha31/8), CD49c (42), CD54 (3E2), CD61 (2C9 G2), CD169 (E50-2440), CC chemokine receptor 5 (C34-3448) from BD Pharmingen (San Diego, CA); major histocompatibility complex class II (28-16-8S) from Caltag Invitrogen (Carlsbad, CA); and CD205 (NLDC-145) from Serotec (Raleigh, NC).



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    Image Search Results


    Mice received anti-CD11a and anti-CD49d antibodies to deplete MZB cells 1 day prior to infection. Subsequently, mice were infected with aerosolized Mtb and consistently received the same dose of depletion antibodies every week until sacrifice. Control mice underwent the same experimental procedure but were given corresponding isotype control antibodies. Mice were sacrificed at 2, 4, 6, and 8 weeks post-infection. (A) Schematic design of experiment. (B and C) MZB cells were gated according to . (B) Frequencies of MZB cells. (C) Counts of MZB cells, n = 8. (D) Bacterial burden in the lungs and spleen, n = 7–8. (E and F) Cytokine patterns in plasma. The levels of cytokines and chemokines were measured by multiplex analysis. (E) Downregulated cytokines in MZB-depleted mice and (F) up-regulated cytokines in MZB-depleted mice, n = 3–8. Mean ± SEM. Error bars are omitted if shorter than the symbols. Unpaired Student’s t test was used to determine the statistical differences between MZB-depleted and control mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001; ns, not significant. The results shown are representative of two independent experiments.

    Journal: Cell reports

    Article Title: Splenic marginal zone B cells restrict Mycobacterium tuberculosis infection by shaping the cytokine pattern and cell-mediated immunity

    doi: 10.1016/j.celrep.2024.114426

    Figure Lengend Snippet: Mice received anti-CD11a and anti-CD49d antibodies to deplete MZB cells 1 day prior to infection. Subsequently, mice were infected with aerosolized Mtb and consistently received the same dose of depletion antibodies every week until sacrifice. Control mice underwent the same experimental procedure but were given corresponding isotype control antibodies. Mice were sacrificed at 2, 4, 6, and 8 weeks post-infection. (A) Schematic design of experiment. (B and C) MZB cells were gated according to . (B) Frequencies of MZB cells. (C) Counts of MZB cells, n = 8. (D) Bacterial burden in the lungs and spleen, n = 7–8. (E and F) Cytokine patterns in plasma. The levels of cytokines and chemokines were measured by multiplex analysis. (E) Downregulated cytokines in MZB-depleted mice and (F) up-regulated cytokines in MZB-depleted mice, n = 3–8. Mean ± SEM. Error bars are omitted if shorter than the symbols. Unpaired Student’s t test was used to determine the statistical differences between MZB-depleted and control mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001; ns, not significant. The results shown are representative of two independent experiments.

    Article Snippet: CD49d antibody (R1-2) , Thermo Fisher Scientific , Cat# 16-0492-85; RRID:AB_468970.

    Techniques: Infection, Control, Clinical Proteomics, Multiplex Assay

    Journal: Cell reports

    Article Title: Splenic marginal zone B cells restrict Mycobacterium tuberculosis infection by shaping the cytokine pattern and cell-mediated immunity

    doi: 10.1016/j.celrep.2024.114426

    Figure Lengend Snippet:

    Article Snippet: CD49d antibody (R1-2) , Thermo Fisher Scientific , Cat# 16-0492-85; RRID:AB_468970.

    Techniques: Blocking Assay, Virus, Purification, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Control, Software, Microscopy

    The initial inferior T cell response to a lower infective dose is better maintained during the chronic stage of infection. C57BL/6 mice were infected via the oral route with 2 or 10 cysts of T. gondii , and the immune response was evaluated at weeks 2, 5, and 8 p.i. ( A and C ) Ag + CD4 T cells (gated on CD4 T cells) and Ag + CD8 T cells (gated on CD8 T cells) were assessed using CD49d/CD11a (A) or CD44/CD11a (C) expression, respectively (week 2 p.i.). ( B and D ) The total numbers of Ag + CD4 (B, CD49d hi CD11a hi ) and CD8 (D, CD44 hi CD11a hi ) T cells were compared in the spleen of infected animals at weeks 2, 5, and 8 p.i. ( E ) The kinetic of KLRG1 + and KLRG1 − CD8 T cells (gated on Ag + CD8 T cells) was evaluated at weeks 2, 5, and 8 p.i. (total number). ( F ) Toxoplasma -specific IgM (week 2 p.i.) and IgG2a (weeks 5 and 8 p.i.) were assessed in the serum by ELISA. ( G ) The parasite burden in the brain of LD- or HD-infected mice was measured by real-time PCR at different time points p.i. * p < 0.05, ** p < 0.01, **** p < 0.001, by a Student t test. Experiments were performed at least three times ( n = 4–6 mice/group), and graphs represent pooled data from three independent experiments (B–G).

    Journal: ImmunoHorizons

    Article Title: A Lower Dose of Infection Generates a Better Long-Term Immune Response against Toxoplasma gondii

    doi: 10.4049/immunohorizons.2300006

    Figure Lengend Snippet: The initial inferior T cell response to a lower infective dose is better maintained during the chronic stage of infection. C57BL/6 mice were infected via the oral route with 2 or 10 cysts of T. gondii , and the immune response was evaluated at weeks 2, 5, and 8 p.i. ( A and C ) Ag + CD4 T cells (gated on CD4 T cells) and Ag + CD8 T cells (gated on CD8 T cells) were assessed using CD49d/CD11a (A) or CD44/CD11a (C) expression, respectively (week 2 p.i.). ( B and D ) The total numbers of Ag + CD4 (B, CD49d hi CD11a hi ) and CD8 (D, CD44 hi CD11a hi ) T cells were compared in the spleen of infected animals at weeks 2, 5, and 8 p.i. ( E ) The kinetic of KLRG1 + and KLRG1 − CD8 T cells (gated on Ag + CD8 T cells) was evaluated at weeks 2, 5, and 8 p.i. (total number). ( F ) Toxoplasma -specific IgM (week 2 p.i.) and IgG2a (weeks 5 and 8 p.i.) were assessed in the serum by ELISA. ( G ) The parasite burden in the brain of LD- or HD-infected mice was measured by real-time PCR at different time points p.i. * p < 0.05, ** p < 0.01, **** p < 0.001, by a Student t test. Experiments were performed at least three times ( n = 4–6 mice/group), and graphs represent pooled data from three independent experiments (B–G).

    Article Snippet: Abs used in the studies were as follows: BioLegend: CD8β (YTS156.7.7), CD4 (GK1.5), CD11a (M17/4), CD44 (IM7), KLRG1 (2F1/KLRG1), TIGIT (1G9), IFN-γ (XMG1.2), CD107a (1D4B), NK1.1 (PB136), CD11b (M1/70), Gr1 (RB6-8C5), CD80 (16-10A1); Thermo Fisher Scientific: CD49d (R1-2), LAG-3 (C9B7W), TIM3 (RMT3-23), TNF-α (MP6-X22), CD11c (N418), CD86 (GL-1).

    Techniques: Infection, Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction